The aim of this course is to provide graduate-level students with a clear and practical understanding of the fundamental principles and applications of chromatographic techniques. It is designed to develop the ability to interpret separation mechanisms, evaluate chromatographic performance, and understand the operational principles of key instruments such as GC and HPLC systems.

Furthermore, the course seeks to equip students with the skills necessary to analyze chromatographic data, optimize separation conditions, and design basic analytical methods for real-world applications in environmental, food, and pharmaceutical analysis.

This course covers the fundamental principles and practical aspects of chromatographic separation techniques at a graduate level. It begins with an introduction to the theoretical foundations of chromatography, including separation mechanisms such as partition, adsorption, ion exchange, and size exclusion, as well as key chromatographic parameters like retention factor, selectivity, resolution, and column efficiency.

The course then focuses on the instrumentation and working principles of major chromatographic techniques, including gas chromatography (GC) and high-performance liquid chromatography (HPLC). Topics include system components, column types, mobile phase selection, injection techniques, and commonly used detectors such as FID, TCD, UV-Vis, and DAD. Data analysis, chromatogram interpretation, calibration methods, and troubleshooting are also addressed.

In addition, the course introduces ion chromatography and size exclusion chromatography, along with essential sample preparation techniques such as extraction, filtration, and derivatization. The final part of the course emphasizes method development and validation, including parameters such as accuracy, precision, linearity, limit of detection (LOD), and limit of quantification (LOQ), and provides an overview of advanced hyphenated techniques such as GC–MS and LC–MS with selected real-world applications.